ABSTRACT
Aim: We aimed to study the expression of CD24 and CD133 in colorectal cancer and normal adjacent tissues to assess a relationship between these markers and clinic-pathological characteristics and patient's survival
Background: Cancer stem cells are a group of tumor cells that have regeneration and multi-order differentiation capabilities
Patients and methods: Expression of CD24 and CD133 was studied in a paraffin block of colorectal cancer and normal tissues near tumors with the immuneohistochemical method in patients who were referred to Imam Khomeini Hospital in Sari
Results: A total of 50 samples [25 males and 25 females] with a mean age of 67.57 +/- 13.9 years old with range 28-93 years, included 3 mucinous carcinoma and 47 adenocarcinoma. Expression of CD133 marker was negative in 29 cases and positive in 21 cases. Expression of CD24 in tissue near tumor cells was found in 30% of available samples. The relationship between expressing CD24 with treatment [surgery and chemotherapy] was significant and its relationship with patient's survival was insignificant statistically. However, there was a clear difference as mean survival age of patients based on CD24 expression was 26.64 +/- 18.15 for negative cases and 41.75 +/- 28.76 months for positive cases. CD24 and CD133 expressions and their co-expression with other clinic-pathological factors were not significant
Conclusion: During this study, the relationship between CD24 and treatment type was significant. To confirm this result, various studies with high sample numbers and other stem cell markers are recommended
ABSTRACT
Background: Some recent studies reported human epidermal growth factor receptor [HER-2/neu] as a marker that can be used in immunological studies of colorectal carcinoma for predicting the prognosis and the treatment. Therefore, we aimed to investigate the frequency of HER-2 expression in patients with colorectal cancer, and explore the relationship between clinicopathological prognostic factors and its expression based on immunohistochemical analysis
Methods: This study included 50 patients with a histologically proven diagnosis of colorectal carcinoma who received surgery at Imam Khomeini Hospital affiliated to Mazandaran University of Medical Sciences. First, HER-2/neu protein expressions were detected by immunohistochemistry and then the data extracted from recorded files
Results: The median age of the patients was 60.2 +/- 13.9 years [range: 25-93 years]. There was no significant relationship between size of tumor, age, sex, lymph node metastases, distant metastasis, differentiation, and stage of the disease with positive expression of HER-2 in this study
Conclusion: No significant relationship between expression of HER-2 and clinicopathological prognostic factors was found in our study. Further comprehensive and prospective trial with standard method to evaluate the role of HER-2 expression among patients with colorectal cancer is needed
Subject(s)
Humans , Male , Middle Aged , Female , Aged , ErbB Receptors , Genes, erbB-2 , Retrospective Studies , ImmunohistochemistryABSTRACT
Esophageal squamous cell carcinoma is a rather common cancer in northern Iran. Incidence of adenocarcinoma of esophagus has an increasing trend in Iran. Co-existence of both cancers in one patient is very rare. We report a middle age woman from northern Iran with a typical presentation of esopha-geal cancer, who was found to have a dual esophageal cancer. The disease was found in the advanced stage with pulmonary metastasis at the presentation. Palliative chemo-radiotherapy induced partial clinical response
ABSTRACT
Cancer stem cells are a subgroup of tumor cells that have the capability for self-renewal and differentiation and are characterized by multiple markers. Several studies have been conducted to identify these cells in colorectal cancer, including stem cell marker CD24. This study has evaluated the association of CD24 expression with clinicopathologic features and survival in an immunohistochemical [IHC] study that compared colorectal cancer tissues with adjacent normal tissues. The expression of CD24 in 50 paraffin embedded samples of colorectal cancer and adjacent normal tissue in patients referred to Imam Khomeini Hospital, Sari, Iran was examined by IHC. We studied the relationship between clinical and pathological features as well as the patient's condition in terms of metastasis, recurrence, type of treatment and death due to cancer treatment. The 50 cases studied included 25 males and 25 females with a mean age of 57.64 +/- 9.13 years range: 28-93 years] There were 3 mucinous carcinoma and 47 adenocarcinoma samples. CD24 was positive in 29 cases and negative in 21 cases. CD24 expression correlated with combined surgery and chemotherapytreatment p=0.04]. A correlation existed with patient survival but it was not statistically significant. The mean survival time of patients according to CD24 expression was 26.64 +/- 18.15 in cases that were CD24 negative and 41.75 +/- 28.67 months in cases that were CD24 positive. Correlation of CD24 expression with other clinicopathologic factors was not significant [p>0.05]. We have observed a relationship between the clinicopathologic feature of combined surgery and chemotherapy treatment to colorectal cancer stem cell marker expression. A nonsignificant correlation also existed with patient survival. To confirm these results, we recommend additional studies with larger sample sizes
ABSTRACT
Liver injury induced by viruses, chemicals and drugs can be protected by different medicinal plants. Feijoa sellowiana [Myrtaceae] is an evergreen bush native to southern areas of South America, as well as Iran where the fruits are very popular. Feijoa has shown a potent antimicrobial effect. Morever, the antioxidant activity of total Feijoa extract has also been reported. MDMA or ecstasy is a ring-substituted amphetamine derivative which has been abused as a widespread recreational drug by the young generation. Liver is a target organ for MDMA toxicity. In fact, this sense MDMA is metabolized by cytochromes P[450]2D, 2B and 3Aand reactive metabolites are readily oxidized to the corresponding o-qiuinones and reactive oxygen species [ROS]
This study investigated whether methanilic Feijoa sellowiana fruits can produce biochemical changes using the Isolated Rat Liver Perfusion [IRLP] system. The, the liver was perfused with different concentrations of the extract [10, 20, 40, 50,100 mg/kg], added to the buffer and perfused within 2 h. During the perfusion we tried to find out the antioxidant activity or liver protective effect of Feijoa, by determinining amino-transferases activities [SGOT and SGPT ] and glutathione reductase [GSH] level in comparison with the positive and negative controls. Subsequently, sections of liver tissue were examined for any histopathological changes. The results revealed that the activities of SGOT and SGPT were seriously decreased and GSH level was significantly increased by the Feijoa extract. Overall, necrosis in the liver parenchyma was decreased. These findings revealed that Feijoa sellowiana is an effective hepatoprotective plant